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Purpose – This study aims to obtain the best lipid content in locally isolated microalga that grows in palm oil mill effluent (POME).

Design/Methodology/Approach – Microalgae were cultured in POME with 25% dilution (LP₂₅), 50% (LP₅₀), and no dilution (LP) in 1,500 ml glass vessel at room temperature using a lighting intensity of 13,000 lux and continuous aeration for 24 hours and 12 hours, respectively. The biomass (in dry weight) of microalgae was analyzed daily by means of spectrophotometry using 624 nm wavelength to determine their growth.

Findings – The results showed that the acclimatized growth of microalgae in POME media adapted faster to the POME concentration. Acclimatized biomass content tends to increase to 1.014 g/L, while the content of non-acclimatized biomass reached only 0.752 g/L. Lipid content resulting from the direct extraction process using the modified Bligh and Dyer method provided the best yield of 47% in the microalgae grown in the LP₅₀ POME medium.

Research Limitations/Implications – Lipid was produced from locally isolated microalgae cultured in POME medium with 25% dilution (LP₂₅), 50% (LP₅₀), and no dilution (LP).

Practical Implications – The lipid produced had the potential for biodiesel energy.

Originality/Value – In this study, microalga was used not only to treat POME liquid wastes but also to produce lipids as biodiesel energy potentials.